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The micro-DBC method (dye binding capacity) developed for single seeds is based on the FP-9 Analyzer System, which was originally developed for medical research. With a few extra devices it was adapted for seed protein micro-DBC determination. With the new analyzing system it is possible to calculate the amount of acid orange 12 dye bound by a single grain, determined per unit weight of the grain. In the FP-9 Analyzer System, nine samples are handled simultaneously at all working stages. The system includes 9-tipped pipettes, a 9-tipped automatic diluter, scales, a grinder for 9 grains, a mixer for Bx 9 samples and a centrifuge for 4x9 samples, and a photometer for 9 samples as well as a programmable desk calculator connected to it. The largest volume to be pipetted is 1 ml. Twentyone barley lines, donated by Dr. L. Hunck, Carlsberg Laboratory, Denmark, were studied with the FP-9 Analyzer System. From each barley line, 4—l2 grains were studied. The correlation coefficient (r) between their means and the results obtained by the macro-DBC system was 0.9600***. The coefficient of variation (C.V. %) within the barley lines was 4.74 23.28 %. With homogeneous standard flour the coefficient of variation was 1.81 3.79 % for eight cuvette blocks with 9 samples each. The micro-DBC system has been specially developed for plant breeding purposes. Screening of material can be carried out on single or half grains in segregating early generations (F 2 —FS). Thus the cultivation of large line materials can partly be avoided.

Application of a semi-automatic micro-DBC analyzer system for screening basic amino acids in single grains