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To explore the optimum concentration and treatment time of colchicine to induce chromosome doubling, the optimal conditions of polyethylene glycol-induced protoplast fusion molecular weight, working concentration and induction time, using orthogonal and single factor, the screening test of high quality characters of lavender polyploidy were carried out. Using colchicine method combined with PEG method for lavender polyploid induction, polyploid plants with excellent traits were selected. Among them, the protoplast fusion rate of PEG with a molecular weight of 6000Da was 19.6% after being induced at 40% for 25 min. Lavender explants were treated with colchicine MS induction medium at a concentration of 0.2% for 48h and the mutagenicity frequency reached 60%. The results showed that the method of polyploid induction of lavender can quickly realize breeding of polyploid varieties of lavender and shorten the breeding cycle, and provide basic data theoretical basis for further study on the industrialized production of lavender and highly oil-resistant polyploid plants.

Research on the Situations of Polyploid Formation of Lavender Induced by Colchicine