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Excessive production of free-radicals may induce oxidative stress, which is the basis of many diseases pathogenesis. Exogenous antioxidant supply is needed to support the function of endogenous antioxidant in preventing oxidative stress. The ethanolic-extract of A.indica and C.asiatica possess the antioxidant activity. This study was aimed to know the antioxidant activity of ethanolic-extract mixture of A.indica and C.asiatica in comparison with ethanolic-extract of A.indica. The combination of extract was expected to improve the antioxidant activity and therefore to reduce the dosage. The 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay test with spectrophotometry was performed to measure the antioxidant activity. This study was conducted at the Department of Pharmacy Faculty of Medicine Universitas Indonesia, from June to October 2015. Vitamin C as the positive control exhibited EC50 score as much as 0.012 mg/mL. The EC50 score of the ethanolic-extract mixture was 13.68 mg/mL, while the ethanolic-extract of A.indica's was 39.65 mg/mL. The lower the EC50 score indicates the higher antioxidant activity. Thus, the ethanolic-extract mixture of A.indica and C.asiatica has lower antioxidant activity compared to the ethanolic-extract of A.indica.

Antioxidant Activity of Ethanolic-Extract Mixture of A.indica and C.asiatica in Comparison with Ethanolic-Extract of A.indica