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The objective of this study is to assess the fungal and antimicrobial activity of the essential oil (ET) of marjoram (Origanum majorana L.). This strategy can contribute to the sustainable development of our country. Preliminary tests performed on the essential oil of Origanum majorana showed that this oil has antibacterial activity vis-à-vis the bacterial strains (Enterococcus feacalis, Enterobacter cloaceai, Proteus microsilis, Escherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa) and antifungic (Fusarium sporotrichioide and Fusarium graminearum). The culture medium used was nutrient broth Muller Hinton. The interaction between the bacteria and the essential oil is expressed by a zone of inhibition with diameters of MIC indirectly expression of. And we used the PDA medium to determine the fungal activity. The extraction of the aromatic fraction (essentially oil hydrolat) of the fresh aerian part of the Origanum majorana was performed by hydrodistillation. The average essential oil yield is 0.99%. The antimicrobial and fungal study of the essential oil and hydrosol showed a high inhibitory effect on the growth of pathogens.

Antibacterial and Antifungal Activity of Essential Oil of Origanum Majorana Lon a Few Bacteria and Fungi