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As the number of rape cases are increasing day by day. There is a requirement of detection of seminal fluid and sperm on various surfaces, even if semen and seminal fluid available in few quantities. The present work focuses to determine three possible aspects affecting semen determination on laundered clothing: the temperature of water while laundering and handling if the stain was dry or wet at the period of laundering of the fabric form. During present work, four types of fabrics (Polyester, Denim, Cotton and wool) were analysed microscopically by applying of methods to determine semen (U.V. light source, Acid phosphatase test) the outcome demonstrates the significance of the methodology and precautions to be taken whenever evaluating findings for the identification of semen, in general on objects which may have been laundered.

Semen Stain Detection: A Study on the Different Fabrics for Effects of Washing