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The experimental anti-AIDS glycerophosphatidic acid:nucleoside (sn-1/sn-2 diacylglycerol:dideoxynucleotide) drugs, 3'-azido-3'-deoxythymidine monophosphate diglyceride ( AZT-MP-DG) [ 1 J and 2', 3 '-dideoxycytidine monophospha te diglyceride (ddC-MP-DG) [2] were isolated and purified by the reversed-phase mode of high performance . liquid chromatography ( RPLC). The chromatograph"ic separation was based upon the glycerophospholipid moiety of the drugs and the detection on the nucleoside component. The separations were optimized on method development columns packed with the stationary phase to be used in the micro-preparative column and monitored by a UV detector. Fractions we re collected and analyzed for purity by analytical-scale high performance liquid chromatography [3] and by thin-layer chromatography [4].

Development of High Performance Liquid Chromatographic Separations for the Purification and Analysis of Phospholipids and Related Liponucleotides - A New Class of Anti-AIDS Drugs