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It is well known that high-strength steel is susceptible to hydrogen embrittlement (HE), where strength or toughness of steel is degraded by hydrogen.1,2) One of reasons for higher HE susceptibility of high-strength steel is likely that high-strength steel, such as martensitic one, generally contains a large number of dislocations, and hydrogen interacts with these dislocations. Thus, in order to suppress HE, elucidation of influence of dislocation density on HE behavior is critical. Regarding the influence of dislocation density, M. Nagumo et al. observed microstructures of pure Fe strained during hydrogen charging, and found that hydrogen nucleates microvoids along deformation bands, where dislocation density is high.3) On the basis of the observed results, they proposed that a linkage of these microvoids results in HE fracture. Y. Todaka et al. investigated HE susceptibility of ultra-low carbon steel with ultrafine grains and different dislocation densities by small punch tests in gaseous hydrogen.4) The samples were made by severe plastic deformation with high-pressure torsion (HPT) and subsequent annealing. They reported that HE occurs for the higher dislocation density sample, whereas HE is suppressed for the lower dislocation density sample. Moreover, D.-H. Lee et al. investigated influence of hydrogen on mechanical properties of linepipe Influence of Hydrogen on Local Mechanical Properties of Pure Fe with Different Dislocation Densities Investigated by Electrochemical Nanoindentation