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The aim of this study was to determine the effect of parachlorophenol (PCP) and camphorated parachlorophenol (CMCP) on nitric oxide (NO) production by a murine macrophage cell line, RAW264.7. The cells were incubated on plastic disks with either PCP or CMCP. Plastic adherent and nonadherent cells were subsequently stimulated with recombinant mouse IFN-gamma or bacterial lipopolysaccharide (LPS). Nitric oxide (NO) levels detected from the culture supernatants were determined by the Griess reaction. The results showed that PCP and CMCP diluted at 10(-1) but not at 10(-3) suppressed NO production by both plastic adherent and nonadherent cells, suggesting that both phenolic compounds may suppress NO production by murine macrophages in a dose-dependent manner.

The effect of parachlorophenol and camphorated parachlorophenol on nitric oxide production by a murine macrophage cell line, RAW264.7.