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The accumulation of mitochondrial DNA mutation is an important contributor to the aging process. Polymerase chain reaction (PCR) amplification was carried out on total DNA from gingival tissues of human subjects with ages of 19 to 64 years, and 5.0-kb and 7.4-kb deletions were found in the mitochondrial genomes of these subjects. This is the first report of mitochondrial DNA deletion detectable in human gingival tissues.

Detection of mitochondrial DNA mutations in human gingival tissues.