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高密度培養したナンノクロロプシスナンノを用いて L 型ワムシの連続培養を行い濃縮淡水クロレラを用いた場合と脂肪酸組成を比較した。その結果ナンノとクロレラともにほぼ同密度でワムシ培養が可能であり餌料効率もほぼ同じであった。クロレラとそれを給餌したワムシには極性脂質が多くナンノとそれを給餌したワムシには中性脂質が多く含まれていた。またクロレラ給餌ワムシではパルミチン酸やリノール酸が多いのに対してナンノ給餌ワムシではイコサペンタエン酸が多くナンノには無いドコサペンタエン酸も少量含まれてい た。   Fatty acid profiles of the L-type rotifer Brachionus plicatilis harvested from a continuous culture system were compared respecting the quality of food algae. Nannochloropsis oculata propagated in high density by a panel type photo-bioreactor and fresh water Chlorella suspension on the market were tested. Continuous rotifer culture was carried out under 24°C and 26 psu in a 2000-mL polyethylene bottle. No difference was observed as to the food efficiency when evaluated from the rotifer density and the daily harvesting rate. Both fresh water Chlorella (FC) and the rotifer which fed on the algae (FC-R) had more polar lipid than non-polar one, whereas both Nannochloropsis (N) and the rotifer which fed on the algae (N-R) had more nonpolar lipid than polar one. FC-R were rich in palmitic acid and linoleic acid, and N-R showed high icosapentaenoic acid (IPA) contents, which may reflect the composition in their food algae. A small amount of docosapentaenoic acid (DPA), however, was detected in N-R, although it was not contained in food (N). It is postulated that the rotifers metabolize IPA into DPA at least in the case when cultured with Nannochloropsis

nihon suisan gakkaishi/nippon suisan gakkaishi

Fatty acid composition of the L-type rotifer Brachionus plicatilis produced by a continuous culture system under the provision of high density Nannochloropsis