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Rainbow trout Oncorhynchus mykiss fingerlings were fed for 15 weeks at 16°C on experimental diets containing different amounts of magnesium L-ascorbyl 2-phosphate (AsP) or L-ascorbic acid (AsA). The fish fed on the AsA-free diet showed external signs of sucrvy at the 9th week and stopped growing at the 12th week. No signs of scurvy were found in fish fed AsP-or AsA-supple-mented diets. The liver and plasma AsA concentrations were slightly higher in AsP-supplemented groups than in the groups supplemented with an equimolar amount of AsA. The molar ratios of hydroxyproline (Hyp) to proline (Pro) of collagens isolated from the skin and bone of the fish fed the AsA-free diet were significantly lower than those from the fish fed AsP- or AsA-supple-mented diets. There were no statistical differences between the ratios of Hyp to Pro for AsP-and AsA-supplemented groups. The foregoing results demonstrate that AsP has an equivalent physiological activity to AsA for rainbow trout.

Utilization of L-Ascorbyl 2-Phosphate in Rainbow Trout as a Dietary Vitamin C Source.