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There is an optimal salt concentration of mating in the conjugal transfer of an R plasmid, and incompatibility (Inc) group C R-plasmids are characteristic in that they demand high salt concentration such as 0.34M or more in the efficient R-transfer. Both sodium and potassium salt are effective but divalent cation (Mg) are not. Many pili-like structure of Escherichia coli carrying an Inc C plasmid Rms 417 were observed on an L agar plate which contained 0.34M salt, but few in the absence of salt. Therefore, the pili could be considered sex pili that E. coli R+ produced in the presence of a moderate amount of monovalent salt.

Efficient conjugal transfer of inc R-plasmids at high salt concentration in the mating media.