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The relative retention value of the individual triglyceride molecular species on reverse phase high performance liquid chromatography was considered to be dependent principally on the addi-tion theorem of chemical potentials of the three fatty acid residues. It was demonstrated that the chemical potential of each fatty acid residue is proportional to the relative retention potential index i. e. the logarithm of the relative retention value of each fatty acid residue; and that the addition of the relative retention potential indexes of the three fatty acid residues denoted the logarithm of the relative retention value, the relative retention time of each individual molecular species of triglyceride. Triglyceride molecular species from sand flounder was analyzed and compared with the theoretically predicted values

Application of partition chromatographic theory for the analysis of marine triglyceride molecular species.