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Kazuomi ITANO,*1 Shin'ichiro KAWAI,*1 and Ryo TATSUKAWA*2 (Accepted November 28, 1984) The salt-insoluble fraction was extracted from the muscle tissue of mature striped dolphins Stenella coeruleoalba, in order to examine the properties of mercury and selenium coexisting in the fraction. After pronase digestion, almost all of the total mercury and selenium in the salt-insoluble fraction were found in a small amount of insoluble residue, although the protein was nearly dis solved. Most of the total mercury and selenium in the insoluble residue were precipitated by centrifugation after solubilization with 0.1M potassium hydroxide solution. The amounts of total mercury and selenium in the above precipitate were almost he same as those in sodium dodecyl sulfate-insoluble fraction of the muscle. By treatment with 2-mercaptoethanol, the amounts of mercury and selenium removed from the salt-insoluble fraction were low, and the molar atio of residual mercury and selenium was approximately 1:1.

Properties of mercury and selenium in salt-insoluble fraction of muscles in striped dolphin.