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The polymannuronic acid 5-epimerase activity was investigated in extracts from five selected species of brown algae, using the carbazole-sulfuric acid method for its detection, and its presence was strongly suggested. The enzyme activity in one of these extracts, that from Ishige sinicola, was investigated in detail by gas liquid chromatography, and decreases during incubation of the M/G ratios of alginate used as substrate were found. The result was an additional evidence for the existence of this enzyme. Using Eisenia bicyclis as an experimental material, it was found that the activity of the enzyme is far higher in the growing regions of the fronds than the matured parts.

Polymannuronic acid 5-epimerase activities in several brown algae and its localization in frond.