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Ovulation in amphibians1-4) can be induced in vitro by the addition of several steroids, such as cortisone, hydrocortisone, androsterone, testosterone and progesterone to Ringer containing the ovarian tissues. In contrast to amphibians, reports on ovulation in vitro in fish with steroids are scarce.5,6) Studies on the effects of steroid hormones in inducing ovulation in vivo in the catfish7-9 suggested that luteinizing hormone (LH) might not act directly on the ovary in the catfish, but via the interrenal gland by inducing the pro duction of the adrenocortical steroids, which were effective for induction of ovulation in the fish. However, the role of steroids in physiological maturation and ovulation in fish has not yet been clear. This report, as the first step to clarify the role of steroid in fish ovulation, describes the effects of several steroids on ovulation in vitro of the Oryzias oocytes.

Biological Study on Ovulation in vitro of Fish-IV