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Adopting the spread agar plate method, several marine phytoplanktons, such as Nitzschia spp., Navicula spp., Amphora sp.(?), and Chlorella sp. were isolated. Among these algae, Nitzschia closterium was made free of bacteia easily on the plate contained 100 and 200 μg/ml streptomycin. This method using a silicon coated glass rod seemed to be preferable to the streaking plate method with a wire loop or a needle.

THE SPREAD AGAR PLATE METHOD FOR ISOLATION AND PURIFICATION OF MARINE PHYTOPLANKTON