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Taken up out of procedures of determinations reported separately by KOMATSU and YASUDA mainly for serum cholesterol, several parts applicable to samples other than serum have been examined experimentally. The results obtained are summarized as follows. 1) When the alkaline solution containing cholesterol, for example the saponification mixture of a sample, is neutralized before addition of digitonin, acetic acid is preferable as a neutralization agent to hydrochloric acid. Strict neutralization is not always essential, so far as acetic acid is used for neutralization. 2) When evaporation of the solvent is carried out to isolate cholesterol dissolved in the mixture of acetic acid and ether according to Yasuda's procedure, it has to be done in the atmosphere of an inert gas such as carbondioxide. 3) Color reaction is performed better by the shaker of compound type (Fig. 1) than by the usual one of single type in regard to intensity, precision of color developed and actual handling.

ON THE COLOR REACTION OF THE DETERMINATION OF CHOLESTEROL BY FERRIC CHLORIDE METHOD AND SEVERAL PRELIMINARY TREATMENT