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TTC reduction by the normal, injured and dead thalli of Porphyra and Monostroma was investigated quantitatively. Thalli of 0.2g. in fresh wt. was immersed in 2mg./cc. (Porphyra) or 4mg./cc. (Monostroma) solution of TTC under incubation for 1 hour at 30°C. under evacuation. The red pigment, formazan, produced by the reduction was extracted with toluence and the optical density was determined at 490 mμ. The activity of formazan production declines when the material have previously been exposed to 10-5 or above of CuSO4 for 15 hours. The decline relates significantly with the grade of injury caused by copper. By naked eye the colour gradation of toluene extracts are also distinguishable. The said quantitative relation supports the validity of the TTC test for discrimination of injury in Porphyra and Monostroma.

Quantitative Relation in the Reduction of TTC by the normal and injured thali of Porphyra and Monostroma