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Quantitave estimation of the amounts of hemoglobin, myoglobin, and cytochrome c in the red and the ordinary muscles of fishes was made, using the pyridine-hemochromogen method of de Duvet5) for hemoglobin together with myoglobin, the method of Drabkin7) for myoglobin and the method of Rosenthal and Drabkin14) for cytochrome c respectively. Results obtained were tabulated in Table 1. In general, the red muscles were found to be considerably richer in these pigments than the ordinary muscles. Particularly the deep-seated red muscle, which is found in fishes such as tunny and bonito, contained a surprisingly large amount of myoglobin, from 1 to 2% on a wet basis.

Chemical Studies on the Red Muscle (“Chiai”) of Fishes-II