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1Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Bunkyo-machi, Nagasaki 852 8521, 2Manten Corporation, Nabeshima-machi, Saga 8490936, 3Analytical Application Engineering Section Tokyo 1, Hitachi High-Tech Science Corporation, Chuo, Tokyo 1040041, 4National Fisheries University, Japan Fisheries Research and Education Agency, Shimonoseki, Yamaguchi 7596595, 5Institute for East China Sea Research, Organization for Marine Science and Technology, Nagasaki University, Taira-machi, Nagasaki 8512213, Japan

Simplification of extraction procedure and evaluation of extraction ratio in pufferfish toxin examination method