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Single-stranded DNA conformation polymorphism (SSCP) analysis of PCR-products obtained by amplification of part of the cytochrome b gene was applied to identify the fish species in canned tuna and sturgeon caviar. Single-stranded DNA was separated by native polyacrylamide gel electrophoresis and visualised by silver staining. Specific patterns of single-stranded DNA (123 nt) were obtained for a number of tunas and bonitos allowing reliable identification of the species being canned. lntra-species variation of the pattern was observed in case of skipjack (Katsuwonus pelamis). Sturgeons caviar was identified by SSCP-analysis of short (123 nucleotides) and long (464 nt) amplicons. Fifteen species of sturgeon could be differentiated, including Huso huso (Beluga), Acipenser aueldenstaedti (Osietra) and A. stellatus (Sevruoa). Strength, limitations and pitfalls of SSCP are discussed.

Application of PCR-SSCP for Fish Species Identification of Canned Tuna and Sturgeon Caviar