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Most of marine invertebrates are osmoconformers, and the osmolality of their body fluids is influenced by the environmental osmolality. Thus, they must adapt to it at a cellular level when they experience change in environmental osmolality. This suggests that marine invertebrate cells are equipped with a specific osmo conforming mechanism that is lading in most of vertebrate Cells. Presently, there has been no reports of the molecular mechanism of cellular adaptation to osmotic stress in marine invertebrates, except studies on amino acid transporters induced by change in environmental osmolality in the marine mussel Mytilus galloprovincialis1) and the pacific oyster Crasotrea gigas2). In this study, to acquire more information of the osmotic responsive mechanism in marine invertebrate cells, we proposed to clone genes specifically induced by change in os<nolahty, by suppression subtractive hybridization in M gnlloprovincialis.

Subtractive cloning of hypo-osmotic responsive gene in Mytilus