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The laboratory stock culture of Chattonella marina harbors several bacterial strains and has been surviving in hundred times of transfer process. As predominant bacteria, Alteromonas, Flexibacter and Pseudomonas spp. were isolated from a non-axenic culture of C. marina. In the state of mixed cultures of an axenic algal strain with co-existent bacterial isolates, the living algal cells were maintained over 30 days after transferred to enrichment sea water (ESS) medium. On the other hand, algal cells began to decrease at 10 days and were not detected at 30 days at all in axenic cultures. On the addition of enrich ment solution (ESP) into axenic cultures, algal cells were stimulated to grow even after reached maxi mum growth. When above 100 mg/l of polypepton was added to non-axenic cultures, the algal cell counts decreased as the total bacterial counts increased. If the supernatant solutions of co-existent bac terial cultures incubated in nutrient rich media (ZE-CI) were added to algal axenic cultures, algal cells were completely killed in above 5% concentrations within 7 days incubation.

Effect of Co-existent Bacteria on the Growth of <i>Chattonella marina</i> in Non-axenic Culture