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Administration of fluoride is known to induce various ultrastructural changes in secretory ameloblasts1-9). One characteristic change occurring in cells is the appearance of globular bodies and large vacuoles that probably originate in secretion granules or the endoplasmic reticulum1-3,8). The true origin of these bodies or vacuoles, however, will remain unsettled until their chemical nature is understood in greater detail. In connection with this, proving the presence in them of an enamel-matrix component should be significant. The present immunocytochemical work has been carried out to detect amelogenins, a major enamel-matrix component, in the aforementioned bodies and vacuoles occurring in rat-incisor ameloblasts after fluoride administration. Materials and Methods Young male Wistar rats, weighing about 100 g each, were injected with 83 mg/kg body weight of sodium fluoride (NaF; Wako Pure Chemicals, Osaka). The drug was dissolved in distilled water and given by single subcutaneous injection. Six hours after administration, the animals were fixed for 15 minutes by perfusion with a mixture of 2% paraformaldehyde and 2.5% glutaraldehyde in a 0.1 M phosphate buffer. After perfusion, the upper incisors were dissected out of the jaws and placed in the same fixative for 2 hours at 4•Ž. They were demineralized in a 10% EDTA solution for 3 weeks and post-fixed in 1% osmium tetroxide for 1 hour at 4•Ž. Following dehydration in a graded ethanol series, the incisors were embedded in epoxy resin (Epok 812, Oken, Tokyo) and ultrasectioned with an LKB microtome (LKB LKB, Bromma). The sections were stained with uranyl acetate and lead citrate for mor-

Demonstration of amelogenins in globular bodies induced in rat-incisor secretory ameloblasts after fluoride administration.