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The purpose of this study was to investigate the proliferation and mineralization activity of the human gingival and periodontal ligament cells in relation to the nature of the dentin surface. Three types of bovine dentin blocks were used: 1) Rp group: dentin block was root planed by hand scaler, 2) CA group : dentin block was treated by citric acid pH 1 for 3 mins and 3) Na group : citric acid treated block was further exposed to 10% Na0Cl for 3 mins. Besides this, hydroxyapatite (HA) blocks were taken as 4 th group. Human gingival cells (HGC) and periodontal ligament cells (HPLC) were seeded on the blocks and cultured. Alkaline phosphatase (ALP) activity and protein concentration of the attached cells on blocks were measured at the 1, 3, 5, 7, 10 and 14 days interval. Furthermore, HGC and HPLC were cultured in DMEM and the blocks were kept on the cell sheet after becoming confluent, media was changed to a-MEM supplemented with 100 nM dexamethasone and 10 mM β-glycerophosphate. Mineralized nodules were stained weekly for 3 weeks by von-Kossa method and the area was measured in relation to the margin of blocks.The results of the protein concentration of the HGC was higher than the HPLC in the 4 groups. Among the 4 groups, protein concentration of HPLC increased at a faster rate in CA group and in HGC, protein concentration increased at a slower rate in Na group. HGC expressed very low ALP activity and was significantly lower than the HPLC. HPLC showed the highest activity in relation to CA group, and lowest activity was observed in relation to Na and HA group. Regarding the mineralized nodule; HPLC formed larger area of nodule than HGC. CA group formed the largest area of nodule in HPLC but there was no difference among the 4 groups in the HGC. The results suggest that the nature of the dentin surface could influence the cellular proliferation and mineralization activity of HPLC, and also the proliferation of HGC. As HGC are able to show mineralization activity in this study though it is very low, it can be suggested that HGC have the potentiality to torm calcitied tissue.

Influence of the Nature of Dentin Surfaces on Proliferation and Mineralization Activity of Gingival and Periodontal Ligament Cells.