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Since SPizizEN reported DNA-mediated transformation in Bacillus subtilis in 19581, many investigators have focused their attention on this phenomenon and evaluated the method as a promissing way for studying fundamental genetics. If one wishes to study transformation through this system, however, one will encounter many difficulties with respect to the technique for preparing bacterial cells which are competent for transformation. In the present paper, some fundamental informations necessary for conducting transformation experiments are given.

DNA-MEDIATED TRANSFORMATION IN BACILLUS SUBTILIS WITH SPECIAL REFERENCE TO THE METHOD FOR PREPARING COMPETENT CELLS