English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Plasmid R100 confers conjugal transfer ability on its host bacteria and is transmitted from one host to another. R 100 and several other conjugative plasmids, such as F and R 1, share structural and functional homology in the tra region which is responsible for DNA transfer (7, 8, 23, 27). The tra region contains at least 26 genes which are considered to be organized into three main operons, traM, traJ and tra Y I(Z) (for recent reviews, see references 18, 36). However, several chromosomal mutations are also known to influence genetic transfer (for a review, see reference 28). For example, transfer of R 100 and F is reduced in a host mutant lacking IHF (integration host factor) (3,10). There are possible IHF recognition sequences in the region containing the origin of transfer, oriT, of R100 (20; see Fig. 1B), but any of these sequences are not actually shown to be bound by IHF. Here we report the determination of the location of the two IHF binding sites in oriT. One site (designated ihfA) was located between the proposed nicking sites and the TraY protein binding site sbyA. The other (designated ihfB) was upstream of the promoter for the traM gene. We discuss the possibility that IHF may be involved in the nicking reaction promoted by a putative TraY-TraI endonuclease by binding at ihfA and regulate the expression of traM by binding at ihfB. To first confirm that IHF actually binds to oriT of R100, a gel retardation assay was carried out by two-dimensional gel electrophoresis. We used an oriT containing plasmid pSI87-XE1(17; see Fig. 1A) digested with XhoII and NsiI. The DNA fragments were incubated with or without IHF and electrophoresed in the first dimension in a polyacrylamide gel, and the gel was treated with SDS and

Binding sites of integration host factor in oriT of plasmid R100.