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Reverse transcriptase sequencing of 16S ribosomal ribonucleic acids of some representatives of the genus Rhodococcus were performed in an attempt to clarify their intra- and inter-generic relationships. The results indicate that the genus Rhodococcus as presently constituted is phylogenetically heterogeneous. On the basis of the 16S ribosomal-RNA sequence and earlier chemical data it is proposed that the genus Gordona (Tsukamura) be revived to accommodate those rhodococcal species containing relatively long-chain mycolic acids (48 to 66 carbon atoms) and MK-9(H2) as the predominant menaquinone type.

Evidence of phylogenetic heterogeneity within the genus Rhodococcus: Revival of the genus Gordona (Tsukamura).