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Nitrosoguanidine-induced manganese-resistant mutants Mnr101, Mnr107, Mnr105, Mnr193, Mnr2-15 and Mnr2-7 of Azospirillum brasilense were isolated and used for associative nitrogen fixation with cheena (Panicum miliaceum L.) genotype RArM6 in acid soil (pH 5.3). Three of the Mn-resistant mutants (Mnr105, Mnr193 and Mnr101) showed crossresistance to aluminium (2.5ppm) and neomycin (15-50μg/ml) but not to streptomycin except Mnr101 and Mnr107. It is also suggested that these three mutants may be most suitable for nitrogen economy in acid soils having various pH and associated factors of acidity.

journal of general and applied microbiology

Manganese-resistant mutants of Azospirillum brasilense: Their growth and relative efficiency in associative nitrogen fixation with cheena (Panicum miliaceum) in acid soil.