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Regarding the plate count of soil bacteria, I showed previously that a 100-fold dilution of nutrient broth (DNB) gave higher counts than the full-strength medium (NB) (1). Bacterial isolates on DNB consisted of two categories; those which grew on NB and those which did not. Bacteria of the latter are oligotrophic and their count can be estimated a s the difference between counts on DNB and NB (2, 3). The present communication describes that higher counts were obtained on DNB solidified with Agar Noble (Difco), Purified Agar (Difco) or Agar Purified (Oxoid) than on the medium with Bacto Agar (Difco) or Wako 1st grade agar (Wako Pure Chemical Industries, Ltd., Osaka). The full-strength nutrient broth contained 10 g each of peptone (Kyokuto Co., Tokyo) and meat extract (Kyokuto Co.), and 5 g of NaC1 in 1,000 ml of deionized water with pH adjusted to 7.0 with 1 N NaOH. DNB was prepared by diluting NB with deionized water. These media were solidified by 1 per cent (wfv) of the agar denoted respectively and sterilized by autoclaving at 120° for 10 min. Plate count was carried out as described previously (3) excepting that plates were incubated for several days in the case of N B and for two weeks or more in the case of DNB.

A note on the effect of different types of agar on plate count of oligotrophic bacteria in soil.