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Micro-metbods for analyses of neutral detergent fiber (NDF), acid detergent fiber (ADF), and permangnnate lignin were compared to the macromethods of Van Soest (1963) and Van Soest and Wine (1%7, 1968). Differences between the two methods were small altbougb the micromethods gave better precision for ADF while the macro-method gave better precision NDF and lignin. Time and reagents needed for analysis were reduced over 60%~ with the micro-digestion methods. In recent years the methods of Van Soest (1963) and Van Soest and Wine (1967, 1968) have become standards for analysis of fiber in forages and ruminant diets. Problems associated with these methods include time required for analysis and investment in equipment. The number of samples that can be digested isseverely limited by the number of digestion and refluxing units. Waldern (1971) modified the macro-digestion procedures of Van Soest (1963) and Van Soest and Wine (1967, 1968) into micro-techniques that require less time, fewer chemicals and less equipment. However, he only evaluated the effectiveness of the micro-procedure with forage samples containing low neutral detergent fiber (<56%) and acid detergent lignin (<7Q/o). The objectives of this study were to compare neutral detergent fiber, acid detergent fiber, and permanganate lignin values determined by a modification of the micro-digestion techniques of Waldern (1971) with the macrodigestion techniques of Van Soest (1963) and Van Soest and Wine ( 1967, 1968) using diet samples from esophageally fistulated cattle. Methods and Materials Diet samples were collected in 1976 on mountain range in northeastern Oregon. The botanical composition of these samples was determined using the procedures of Sparks and Malechek (1968). Five diet samples were chosen that represented different forage classes and seasons. In late spring (June 20-July 18) on northeastern Oregon mountain ranges forages are immature and highly digestible. Grasses and forbs are the major diet constituents (HoleA$ors, at the time of the research, were graduate assistant and professor ofrange ootr~ttoo. Oregon State University, Eastern Oregon Agricultural Research Center, Union 978?3. J.L. Holechek is currently assistant professor of range science, &partment of Ammalaod Range Sciences. New Mexico State University, LasCruces 88003. This paper is Oregon State Agricultural Experiment Station Technical paper Number 5552. JOURNAL OF RANGE MANAGEMENT 3!5(6), November 1962 799 chek 1980). In late summer (August 15September 14) diet shifts occur where availability or alternate forages allow. Cattle often consume shrubs in late summer and fall (September 14-October 12). Therefore, diets analyzed were high grass and high forb, late spring; high grass and high shrub, late summer; and high browse, fall. “High” refers to the diet constituents that comprised 40% or more of the total diet. Fifteen sub-samples from each of the 5 selected esophageal fistula samples were digested by the microdigestion (Waldern 1971) and standard Van Soest (1963) and Van Soest and Wine (1967 1968) digestion procedures. Differences between the two methods were tested using a paired I-test according to Steel and Torrie (1960).

Comparison of Micro- and Macro- Digestion Methods for Fiber Analysis