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The following description is based mostly on living material except for the measurements, which were all done on metacercariae freed from their cysts, fixed in hot AFA, and stained with celestin blue B. The outer host cyst is heavily pigmented, is nearly spherical, and ranges from 472 to 576g in diameter. The inner thin cyst, which is probably of parasitic origin, is also nearly spherical and measures between 202 and 324/i in diameter. It cannot be separated easily from the host cyst as is the case with Uvulifer (Neascus) ambloplitis. However, the metacercaria itself of N. nolfi is more easily freed from the cyst with the use of small forceps and needle. The metacercaria, which is clearly divided into fore and hind bodies, measures 290382A (340 average of 4) in length and 137-153/i (147 average of 4) in greatest width. The forebody, which is thin, flat and leaf-shaped with posterior edges forming a shallow cup like most Diplostomatidae metacercariae, averages 229/i in length by 147/i in greatest width. In living worms, the great width of the forebody is very striking even while still in the cyst. The conical, rounded hindbody measures 61-107/i in length by 52-61/i in greatest width. The oral sucker is 44/i wide x 41/i long and faces anteroventrally. No prepharynx could be seen. The pharynx measures 13 x 12/i and' the esophagus is 24 to 27A long. The intestinal crura can be seen extending into the hindbody in stained specimens but the terminations could not be ascertained; they could not be seen at all in unstained worms. The ventral sucker, which is located about mid-way in the forebody, is 17A in diameter. The round holdfast organ is approximately 55/i in diameter. In no instances could a reserve bladder of the excretory system be discerned; this is unusual in a Neascus. There is little likelihood that these are "young" forms of other Neascus species in which the reserve bladder system has not yet developed, because the host cyst is already pigmented. In the only black-spot Neascus which has been studied experimentally, the metacercaria is fully developed by the time the cyst becomes melanated (Hunter and Hamilton, 1941). No evidence of reproductive fundament or holdfast gland could be discerned.

Neascus nolfi n. sp. (Trematoda: Strigeida) from Cyprinid Minnows with Notes on the Artificial Digest Recovery of Helminths