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It has been shown that the fertilization membrane of the Urechis embryo can be removed by treatment with a sucrose-EDTA solution. The resulting membraneless embryos appear to undergo nearly normal embryogenesis, differing from the controls only in shape, degree of motility and ability to form double-embryos.

REMOVAL OF THE FERTILIZATION MEMBRANE OF FERTILIZED EGGS OF URECHIS CAUPO AND DEVELOPMENT OF "MEMBRANELESS" EMBRYOS