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A new method of bioassay for antidiuretic substance was reported. Assay was carried out with help of an apparatus for indicating automatically the time of natural urine voiding of several rats and the test material was injected intravenously into non-anesthetized rat.Antidiuretic response was expressed as the antidiuretic effect on a constant water diuresis, which could be expected for forty minutes.Antidiuretic activity of the plasma in the normal subjects was quite slight in the early morning, while significantly increased in the midday and tended to decrease in the evening.

A NEW METHOD OF BIOASSAY FOR ANTIDIURETIC SUBSTANCE AND ANTIDIURETIC ACTIVITY OF THE PLASMA IN THE NORMAL SUBJECTS