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In this work there was evaluated the method of detection of methicillin   resistant Staphylococcus aureus (MRSA) by using two molecular and three   phenotypic tests in investigation procedure of 70 strains of S.aureus   isolated from animals. Recent findings of the new mecA homologue, mecALGA251,   minimise the significance of mecA gene presence detection as a confirmation   method of methicillin resistant Staphylococcus aureus identification. For   this reason, along with multiplex PCR set of primers(165rDNK, nuc, mecA) for   detection mecA gene, there was also used multiplex PCR set of primers (spa,   mecA, pvl, mecALGA251) for differentiation mecALGA251 from mecA, with   simultaneous detection of luk-PV and spa gene fragments. In all 70   investigated isolates there was detected the presence of specific 16 SrDNK   fragment and nuc gene which encodes a thermostable S. aureus nuclease, while   in 5 out of 70 S. aureus isolates, there was proven mecA gene presence using   two multiplex PCR tests. In the investigated strains there was determined   neither mecC (mecALGA251)gene presence, nor Panton Valentine Leukocidin   encoding gene. By application cefoxitin disk-diffusion, latex-agglutination   and two multiplex PCR tests, the identical results in identification 5   methicillin resistant out of 70 investigated S. aureus strains were obtained.   In our investigation there was determined a complete correlation between the   results of phenotypic and genotypic identification of methicillin resistant   S. aureus. [Projekat Ministarstva nauke Republike Srbije, br. TR 31079

Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals