English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Antibiotic residues when present in animal tissues, through food chain, can   enter human body, causing allergic reactions or facilitating the development   of resistant bacterial strains. In order to determine the presence of   antibiotics in animal tissues, it is appropriate to use convenient, reliable   and sensitive methods. Microbiological methods applied for the detection of   antibiotic residues in primary products of animal origin are based on the   sensitivity of specific bacterial strains to a particular group of   antibiotics. Regulatives on the amount of pesticides, metals and metalloids   and other toxic substances, chemotherapeutics, anabolics and other substances   which can be found in food ("Off. Gazette", No. 5/92, 11/92 - corr. and   32/02), state that milk and milk products can be used in commercial purposes   only if not contain antibiotics in quantities that can be detected by   reference methods. The applied method is modified STAR (Screening test for   detection of antibiotics) protocol, regulated by the CRL (Community Reference   Laboratory) Fougeres, France, in which the initial validation of the method   had been carried out. In accordance with the demands of Regulative Commission   EC No657/2002, the sensitivity of modified STAR protocol for beta lactam   antibiotics group was examined , that is, there was carried out a contracted   validation of the method, which initial validation had been performed at CRL.   In a couple of series of experiments, 20 blank samples of raw cow milk   originating from animals not treated by antibiotics, had been examined. By   the beginning of the experiment samples were stored in a freezer at -20ºC.   Samples of raw cow milk enriched by working solutions of seven beta-lactam   antibiotics, in order to obtain concentrations at the level of 0.5, 1 and 1.5   MRL (Maximmum Residue Limit) for each given antibiotic (Commission Regulation   EC No. 37/2010). For detection of beta-lactam antibiotics, there was used   Kundrat agar test with previously inoculated G.stearothermophilus ATCC 10149   strain. Aliquots of 30 _l of working solution at 0.5, 1 and 1.5 MRL   concentration level, for each antibiotic, were inflicted on two paper disks   placed on inoculated Kundrat agar surface. Petri plates with Kundrat agar   previously inoculated with G.stearothermophilus , on which the samples were   deposited, were incubated for 12-15h at 55oC. The obtained width of   microorganisms growth inhibition zone, that is supposed to be at least 2.0   mm, measured from the disc edge, demonstrated the capability to detect all   the tested 7 antibiotics from the beta lactam group at a level below the   MRLs. Consequently, this proves that use of this method it is possible to   meet the demands of Regulative Commission EC No. 37/2010. [Projekat   Ministarstva nauke Republike Srbije, br. III-46009

Evaluation of sensitivity of modified star protocol microbiological method for beta-lactame antibiotics detection in raw cow milk