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Bovine herpesvirus 1 (BHV-1) is one of the most siginificant causes of   infections of the respiratory tract of cattle and immunoprophylaxis has a key   role in curbing this infection. The intensity of the immune response against   BHV-1 following immunization using inactivated commercial vaccines varies   depending on the type of vaccine, but it is generally believed that they   provide good protection from the development of the clinical form of the   infection, and that they are safe. The paper present the development of the   humoral immune response in fattening calves that were immunized against   bovine herpesvirus 1 (BHV-1) at different time intervals between the primary   and the secondary immunization. Calves were administered a commercial   vaccine, and then they were divided into two groups which were revaccinated   on days 14 or 21. Over a course of the 120 days of the duration of the   experiment, blood and nasal mucus were sampled 11 times. The blood serum   samples were examined for antibodies to BHV-1 using the virus neutralization   (VN) test, and the nasal mucus samples were analyzed using the VN test and   the ELISA method. Following revaccination, it was established that there was   an increase in the antibody titer in blood of all experimental animals, and   it was maintained at a high level up until the very end of the experiment   (day 120). In the blood serums, maximum mean values for the antibody titer   were determined on day 30 in the group that was revaccinated on day 14, and   on day 45 in the group of calves revaccinated on day 21. In nasal mucus,   antibodies were established at the earliest, using the virus neutralization   test, on day 14 following vaccination, and using the ELISA method only after   revaccination. The highest antibody titer in nasal mucus was established on   day 45 in the group revaccinated on day 21, and on day 120 in the group   revaccinated on day 14. Based on the established antibody titer values,   calves can be revaccinated using the inactivated BHV-1 vaccine already on day   14. [Projekat Ministarstva nauke Republike Srbije, br. 31084

veterinarski glasnik

Comparative investigations of immune response of calves at different intervals between primary and secondary immunization using inactivated bovine herpes virus 1 vaccine