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This paper describes paratuberculosis in an isolated breeding herd of 25   high-yield dairy cows of the Red Holstein breed. The animals were examined   clinically and then given the test for ldelayed type hypersensitivity and   their blood serum was examined for the presence of specific antibodies   against Mycobacterium avium subsp. paratuberculosis (Map). The clinical   examination revealed that two cows exhibited symptoms of the disease that   indicated an advanced stage of paratuberculosis. The following parameters   were examined in the blood of the cows that showed clinical signs of the   disease: leukocytes and erythrocytes count, concentrations of total proteins,   albumin, iron, sodium, potassium, and activity of creatine kinase. The   analysis of the red blood cell count revealed certain digressions that   indicated the existence of hypochromic microcytic anaemia. The number of   leukocytes was within the physiological values, but the neutrophil-lymphocyte   ratio was disrupted and stood at almost 1:1. The results of the biochemical   analyses of the blood serum of diseased cows indicated hypoproteinaemia,   hypoalbuminaemia, hypoferremia, hyposodiumaemia, hypokalemia, and increased   activities of creatine kinase enzymes. A suspect reaction on the site of   application of avian tuberculin was determined in two animals. Animals with   clinical signs of the disease reacted negative to the test of delayed type   hypersensitivity. The presence of specific antibodies against the cause of   paratuberculosis was proven in four animals (16%), including two animals with   clinical signs of the disease and one that had a suspect reaction on the site   of application of avian tuberculin. Furthermore, one animal that died   exhibited macroscopic and microscopic changes regarding the intensity and   distribution of lesions, the type of cellular infiltrate, and the number of   present acidresistent bacteria, and the changes were characterized as diffuse   changes of multibacillary type. The cause of bovine paratuberculosis was   isolated from the altered organs

Paratuberculosis in breeding stock of red Holstein cows