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Walnut (Juglans regia) is an important economic tree specie, and has unique  aroma in its leaf. In order to investigate the bioactive and bioenergy  characteristic of compounds in ethanol and benzene extracts from fresh  walnut leaf by gas chromatography/mass spectrometry. The results indicated  that ethanol and benzene extracst fresh walnut leaf contain abundant  alcohols, aromatic hydrocarbons, quinones, phenols, aldehydes, acids,  ketones, alkanes, esterscompounds, especially including  dl-.alpha.-Tocopherol, juglone, squalene andlupeol, which areimportant  bioactive components. The functional analytic result suggested that  compounds from fresh walnut leaf extractscan be developed into raw materials  for industries of biofuel, biomedicine, cosmetic, spices and food additive.  The ethanol extract of fresh walnut leaf is more rich in biomedicine and  food additives components, such as ketones, alkanes, aldehydes, amines and  acides compounds. The benzene extract of fresh walnut leaf is more rich in  biofuels, spices, and cosmetic components, such as phenols, aromatic  hydrocarbons, olefines and quinones compounds.

Characterization of bioactive and bioenergy components from fresh walnut (Juglans regia) leaf