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. The prevalence of dental diseases in India is increasing.  Antibiotic resistance exhibited by microbes has paved way for alternative  therapy such as phytotherapy. Pomegranate (Punica granatum Linn) has been  used as an alternative therapy in the treatment of many infections. The aim  of this study was to assess polyphenol content and minimum inhibitory  concentration (MIC) of different parts of pomegranate against oral bacteria.  Materials and Methods. Methanolic and aqueous extracts of seed, pith, rind  and crude extract of pomegranate were prepared. Total polyphenol content was  estimated by Folin-Ciocalciteu reagent using gallic acid as standard. MIC was  assessed for four bacterial species and one fungal species. Results.  Methanolic and aqueous extract of pomegranate pith showed high polyphenol  content (805.6 g/meq of gallic acid) compared to other pomegranate part  extracts. Methanolic and ethanolic seed extracts were effective against  Staphylococcus aureus, crude extract of pomegranate was effective against  Staphylococcus epidermis whereas methanolic and ethanolic rind extracts of  pomegranate were effective against Pseudomonas aeruginosa and Candida  albicans. Conclusion. All parts of pomegranate contain high polyphenol  content and have antibacterial and antifungal activity. Pomegranate can be  potential substitute for synthetic antibiotics against oral microorganisms.

Total polyphenol content and minimum inhibitory concentration of pomegranate (Punica granatum Linn) extracts against oral microorganisms