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Antifungal activity of thyme essential oil against Monilinia fructigena and  development of an effective and stable oil formulation for agricultural use  were studied in this paper. Bioactivity of the oil itself and its developed  formulation, an emulsifiable concentrate (EC), was tested in vitro and in  vivo. In vitro experiments, using a slightly modified agar overlay technique,  showed that the initially emulsified thyme essential oil, as well as the  developed formulation, significantly inhibited mycelial growth of M.  fructigena in vitro. Experiments in vivo, performed on inoculated apple  fruits, revealed that the formulation successfully decreased oil evaporation  from the treated area and provided a significant level of M. fructigena  suppression, 64.7-72.1% compared to the control. To our knowledge, an EC  formulation of thyme essential oil for agricultural uses had never been  developed before. The presented results are initial findings and evaluation  of product activity should be continued in the field to determine its  efficacy and activity spectrum, and to estimate the economic aspect of its  use. [Projekat Ministarstva nauke Republike Srbije, br. III 46008]

Development of a thyme essential oil formulation and its effect on Monilinia ructigena