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Interpretive retention modeling was utilized to optimize the isocratic ion   chromatographic (IC) separation of the nine anions (formate, fluoride,   chloride, nitrite, bromide, nitrate, phosphate, sulfate, oxalate). The   carbonate-bicarbonate eluent was used and separation was done on a Dionex   AS14 ion-exchange column. The influence of combined effects of two mobile   phase factors, the total eluent concentration (2 - 6 mM) and the   carbonate/bicaronate ratio from 1:9 to 9:1 (which corespondent to pH range   9.35 - 11.27), on the IC separation was studied. The multiple species   analyte/eluent model that takes into account ion-exchange equilibria of the   eluent and sample anions was used. In order to estimate the parameters in the   model, a non-linear fitting of the retention data, obtained at two-factor   three-level experimental design, was applied. To find the optimal conditions   in the experimental design, the normalized resolution product as a   chromatographic objective function was employed. This criterion includes both   the individual peak resolution and the total analysis time. A good agreement   between experimental and simulated chromatograms was obtained. [Projekat   Ministarstva nauke Republike Srbije, br. III43009

Interpretative optimization of the isocratic ion chromatographic separation of anions