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Biogenic amines are integral part of nearly every cell. In present study, we   used method of acidic extraction of histamine (His), of polyamines putrescine   (Put), spermidine (Spd) and catecholamines epinephrine (Epi) and   norepinephrine (NE) from human serum; precolumn derivatization with dansyl   chloride, and LC/DAD analysis of the biogenic amines, in aim to monitor   differences of their levels in patients with diabetes mellitus, chronic   urticaria, and Hashimoto's thyroiditis, compared to healthy subjects, and to   observe them as possible markers for immune mediated diseases. Method of   retention times was used for determination of serum biogenic amines. We found   statistically significant differences in putrescine and histamine levels in   diabetes mellitus patients; putrescine, histamine, spermidine and epinephrine   levels in chronic urticaria patients compared to healthy controls, and   putrescine and spermidine levels in Hashimoto's thyroiditis patients,   compared to controls. Norepinephrine was found only in serum of patients with   chronic urticaria. The values of recovery, evaluated in controls, varied   between 85.7% and 106.7%. The statistically significant changes in   putrescine, histamine, spermidine and epinephrine levels in patients compared   to healthy people reflects the existence of biochemical disturbances in   mentioned immune-mediated diseases

LC/DAD determination of biogenic amines in serum of patients with diabetes mellitus, chronic urticaria or Hashimoto's thyroiditis