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Triticale (Triticosecale, Wittmak) an important industrial crop, with high   grain yield, containing high amounts of starch, proteins and also major and   minor mineral elements, is not sufficiently utilized yet. The simultaneous   production of α-amylase and glucoamylase isoforms by Aspergillus niger on   triticale grains, without any nutritive supplements, has been developed,   optimized and scaled up 10 fold for the first time. The specific combination   of examined effects led to the production of a novel glucoamylase isoform.   Reduction of particle size, increase of oxygen availability and substrate   height lead to increase of amylases production of 30%. Reduction of relative   humidity from 65% to 30% increased glucoamylase production 2 times and   α-amylase production for 30%. Peak production of α-amylase (158 U/g) and   glucoamylase (170 U/g) were obtained in erlenmayers and in scaled-up trays.   The obtained A. niger amylase cocktail was more efficient in raw starch   hydrolysis from wheat flour, 29% more efficient in glucose formation and 10%   more efficient in total reducing sugar formation, than commercially   available amylase cocktail SAN Super 240L widely used in industry. [Projekat   Ministarstva nauke Republike Srbije, br. 172048

Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate