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The purpose of this study was to evaluate the antioxidant potential of  Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in  distilled water and partitioned with n-hexane, chloroform, ethyl acetate and  nbutanol sequentially. Phytochemical screening showed presence of phenolics,  flavonoides and cardiac glycosides in large amount in chloroform, ethyl  acetate and n-butanol fraction. The antioxidant potential of all these  fractions and remaining aqueous fraction was evaluated by four methods:  1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total  antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and  ferric thiocyanate assay along with determination of their total phenolics.  The results revealed that ethyl acetate soluble fraction exhibited highest  percent inhibition of DPPH radical as compared to other fractions. It showed  81.14 ± 1.38% inhibition of DPPH radical at a concentration of 60 μg/ml. The  IC50 of this fraction was found to be 33.95 ± 0.58 μg/ml, relative to  butylated hydroxytoluene (BHT), having IC50 of 12.54 ± 0.89 μg/mL. It also  showed highest FRAP value (380.53 ± 0.74 μM of trolox equivalents) as well as  highest total phenolic contents (208.58 ± 1.83 GAE μg/g) and highest value of  inhibition of lipid peroxidation (58.11 ± 1.49% at concentration of 500  μg/ml) as compared to the other studied fractions. The chloroform fraction  showed highest total antioxidant activity i.e.1.078 ± 0.59 (eq. to BHT)

Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa