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The anti-oxidative and antimicrobial activities of different extracts from Hieracium pilosella L. (Asteraceae) whole plant were investigated. The total dry extracts were determined for all the investigated solvents: methanol, dichloromethane, ethyl acetate and dichloromethane:methanol (9:1). It was found that the highest yield was obtained by extraction with methanol (12.9 g/100 g of dry plant material). Qualitative and quantitative analysis were performed by the HPLC method, using external standards. Chlorogenic acid, apigenin-7-O- -glucoside and umbelliferone were detected in the highest quantity in the ex- tracts. The qualitative and quantitative composition of the extracts depends on the solvent used. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect of the extracts was determined spectrophotometrically. The highest radi- cal scavenging effect was observed in the methanolic extract, both with and without incubation, EC50 = 0.012 and EC50 = 0.015 mg ml -1 , respectively. The antimicrobial activities of the extracts towards the bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Klebsiella pneumoniae) and the fungi (Aspergillus niger and Can- dida albicans) were determined by the disc diffusion method. The minimal in- hibitory concentrations were determined for all the investigated extracts against all the mentioned microorganisms.

Anti-oxidative and antimicrobial activities of Hieracium pilosella L. extracts