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Ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLME)   coupled with high performance liquid chromatography (HPLC)-UV detection was   developed for simultaneous extraction and determination of nickel, cobalt,   copper and zinc ions. In the proposed approach, salophen   (N,N'-bis(salisyliden)-1,2-phenylenediamine) was used as a chelating agent;   the ionic liquid, 1-hexeyl-3-methylimidazolium hexafluorophosphate, and   acetone were selected as extracting and dispersive solvents, respectively.   After extraction, phase separation was performed by centrifugation and the   sedimented phase (ionic liquid) was solubilized in acetonitrile and directly   injected into the HPLC for subsequent analysis. Baseline separation of metal   ion complexes was achieved on a RP-C18 column using a gradient elution of the   mixtures of methanol-acetonitrile-water as the mobile phase at a flow rate of   1.0 mL min-1. The influence of variables such as sample pH, concentration of   the chelating agent, amount of ionic liquid (extraction solvent), disperser   solvent volume, extraction time, salt effect and centrifugation speed were   studied and optimized. Under the optimum conditions, the enrichment factor of   222 was obtained. The detection limits for Ni, Co, Cu and Zn were 0.8, 1.6,   1.9 and 2.8 μg L−1, respectively. The relative standard deviations (RSDs)   were in the range of 3.6-5.0 % for all of the investigated metal ions. The   proposed procedure was successfully applied to the determination of the   studied metal ions in water samples

journal of the serbian chemical society

Ionic liquid-based dispersive liquid-liquid microextraction combined with high performance liquid chromatography-UV detection for simultaneous preconcentration and determination of Ni, Co, Cu and Zn in water samples