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Extraction of plant secondary metabolites is an essential step in isolation   of natural products. Non-optimized extraction conditions can lead to losses,   degradation and modification of the biomolecules. In this paper, the   influence of different solvent mixtures, solvent amounts, temperature,   extraction time, and procedures for defatting on yield and profile of various   classes of secondary metabolites was investigated. Rumex alpinus was used for   the extraction of anthraquinones, Glycine max for isoflavonoids,   Chaerophyllum bulbosum for flavonoids and phenolic acids, Anthriscus   sylvestris for lignans and coumarins, alkaloids were extracted from Lupinus   albus and sesquiterpene lactones from Artemisia absinthium. Extraction   efficiency was evaluated by use of LC-DAD-ESI-MS/MS. The compromise   extraction solvent for all of the examined compounds is 80 % methanol, mixed   in ratio 13 : 1 with plant material. Maceration should last for six hours,   repeated four times with fresh solvent. Defatting of the extracts does not   lead to significant losses of the compounds of interest. It is acceptable to   use extraction and evaporation temperature of 60ºC, while the extracts should   be stored in the dark, on -20ºC. [Projekat Ministarstva nauke Republike   Srbije, br. 172058

Optimization of extraction conditions for secondary biomolecules from various plant species