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New, simple, cost effective, accurate and reproducible RP-HPLC method was   developed and validated for the quantification of trans-resveratrol in the   extracts of grape exocarp and seeds. The method has proved to be simpler and   faster than available methods. Methanol was used as a mobile phase with a   flow rate of 1.0 cm3 min-1, while the quantification was effected at 306 nm.   The separation was performed at 35°C using a C18 column. The results showed   that the peak area response was linear in the concentration range of 1-40 μg   cm-3. The values of LOD and LOQ were found to be 0.125 and 0.413 μg cm-3,   respectively. The antioxidant activity of the extracts was determined using   DPPH assay. The ability of DPPH radicals inhibition decreases in the   following order: the extract of grape exocarp > trans-resveratrol standard >   the extract of grape seeds. [Projekat Ministarstva nauke Republike Srbije,   br. TRp-34012

Development and validation of an RP-HPLC method for quantification of trans-resveratrol in the plant extracts